Frequently asked questions
My email validation link is too old, how can I gate a new one?
Go to the Login webpage and request a new email activation link.
Where should I go to buy tickets?
To buy tickets, you first need to have a validated account. You thus need to Register, Validate your email and wait for your account activation by the Genomnis team.
I am not able to validate my email, what should I do?
1. When you click on the link to validate your email, it automatically opens the corresponding page in your default browser. if this browser is too old, it may not be compatible with the recent html standards used in HSF Pro.
How can I register to HSF?
To register to use HSF, please go to the "HSF" menu and select the "Access HSF" sub menu. Here you will either be able to register or to login.
Who is allowed to get free academic tickets?
Only academic institutions performing research can be granted the "academic" status. People working in Hospitals cannot be granted this status.
How can I interpret the HSF-Pro results?
To understand the various HSF-Pro scores, you can read the HSF original publication. In summary you can apply the following rules:
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Splice sites -
Each mutation predicted to affect a splice site is characterized by 3 scores: the wt CV (consensus value), the mutant CV and the ∆CV. -
A sequence is considered as a potential splice site if its CV is superior to 60 using the HSF splice site matrices. You can consider that splice sites with a value of 60-80 correspond to weak sites; 80-90 to average sites; >90 to strong sites. For MaxEntScan, a weak site will have a value of 3-6; a medium site 6-9 and a strong site >9. -
Nevertheless, the most important value is not the mutant CV but the ∆CV that should be >+/-10% to result in the disruption or the creation of a splice site. -
For example a mutation transforming a wt CV of 100 to a mutant CV of 85 (∆CV=-15%) should be considered as a mutation with a strong impact on the corresponding splice site even if the mutant CV of 85 correspond to a strong splice site.
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This information is not sufficient to conclude about the possible impact on the splice site. You also need to consider if the mutation impacts the acceptor or the donor splice site. -
For the donor splice sites, there is no rule and every prediction should be considered for confirmation at the RNA level. -
For acceptor splice sites, you need to consider the position of the mutation. In fact you have to keep in mind that the Branch point will select the closest 3' acceptor site (with a minimal distance between the 2 of around 20 bases). -
If your mutation creates a new splice site between the Branch point and the wt site, it will most probably be chosen (if the distance is ok) -
If your mutation creates a new splice site donstream of the wt site, it will most probably not be used by the cell except in a very specific situation when the mutation also disrupt the ESR balance to silence the wt site.
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Branch Points -
HSF-Pro has already computed all natural branch points for all exons. Only mutations significantly disrupting those signals will be displayed.
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ESE-ESR -
HSF-Pro now provides an integrated vision of all auxiliary signals potentially modified by a mutation. For more information, please go here.
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